Primer and Barcode Gap Identification and DNA Barcode Generation for Species Discrimination in Plants
نویسندگان
چکیده
منابع مشابه
A DNA barcode for land plants.
DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 g...
متن کاملRefining the DNA barcode for land plants.
T he goal of DNA barcoding is conceptually simple: Find one or a few regions of DNA that will distinguish among the majority of the world’s species, and sequence these from diverse sample sets to produce a large-scale reference library of life on earth (1). This approach can then be used as a tool for species identification and to help in the discovery of new species (2). Since the first DNA ba...
متن کاملIdentification of Fabaceae plants using the DNA barcode matK.
In this study, we tested the applicability of the core DNA barcode MATK for identifying species within the Fabaceae family. Based on an evaluation of genetic variation, DNA barcoding gaps, and species discrimination power, MATK is a useful barcode for Fabaceae species. Of 1355 plant samples collected from 1079 species belonging to 409 diverse genera, MATK precisely identified approximately 80 %...
متن کاملAn oligonucleotide barcode for species identification in Trichoderma and Hypocrea.
One of the biggest obstructions to studies on Trichoderma has been the incorrect and confused application of species names to isolates used in industry, biocontrol of plant pathogens and ecological surveys, thereby making the comparison of results questionable. Here we provide a convenient, on-line method for the quick molecular identification of Hypocrea/Trichoderma at the genus and species le...
متن کاملInternal Transcribed Spacer (its), an Ideal Dna Barcode for Species Discrimination in Crawfurdia Wall. (gentianaceae)
BACKGROUND DNA barcoding is a technique used to identify species based on species-specific differences in short regions of their DNA. It is widely used in species discrimination of medicinal plants and traditional medicines. MATERIALS AND METHODS In the present study, four potential DNA barcodes, namely rbcL, matK, trnH-psbA and ITS (nuclear ribosomal internal transcribed spacer) were adopted...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: SSRN Electronic Journal
سال: 2015
ISSN: 1556-5068
DOI: 10.2139/ssrn.3580017